Andy Fluor™ 350 Goat Anti-Mouse IgG (H+L) Antibody

Introduction

Andy Fluor™ 350 Goat Anti-Mouse IgG (H+L) Antibody is prepared by labeling high quality goat anti-mouse IgG (H+L) with Andy Fluor™ 350. Andy Fluor™ 350 is a blue fluorescent dye spectrally similar to the traditional blue fluorescent dye, AMCA. However, compared to AMCA, Andy Fluor™ 350 is more water-soluble, more photostable and more fluorescent on proteins. Andy Fluor™ 350 can be used as a replacement for Alexa Fluor® 350.

Reconstitution and Storage: Store lyophilized power at 2-8ºC. When ready to use, rehydrate with dH2O (50 µL for 100 µg antibody or 250 µL for 500 µg antibody) to make 2 mg/mL solution and centrifuge if not clear. Product is stable for about 6 months at 2-8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.

Specifications   Form Information
Host/Isotype: Goat   Physical State: Lyophilized power
Class: Polyclonal   Shipping Condition: Ambient
Type: Secondary Antibody   Purification: Immunoaffinity chromatography
Tested Species Reactivity: Mouse (Mu)   Buffer: PBS, pH 7.4, with 0.1% BSA
Target Class: IgG (H+L)   Preservative: 0.02% sodium azide
Antibody Form: Whole Antibody   Storage Conditions: 4ºC; store in dark
Label: Andy Fluor™ 350    
Excitation/Emission: 345/440 nm

 

 

Immunofluorescent stain of α-tubulin with Andy Fluor™ 350 Goat Anti-Mouse IgG (H+L) Antibody

 
Unit Size: 100 µg, 500 µg
   
Applications Dilution
Western Blot (WB) 1:5,000 – 1:20,000
Immunofluorescence (IF) 1:100 – 1:2,000
Immunocytochemistry (ICC) 1:100 – 1:2,000
Immunohistochemistry (IHC) 1:100 – 1:2,000
Flow Cytometry (FACS) 1:100 – 1:1,000
   

 

To Order

Buy Cat.No. Product name Ex/Em (nm) Size Price(¥)

L100A
L100B
Andy Fluor 350 Goat Anti-Mouse IgG (H+L) Antibody 345/440 100 µg
500 µg
300
600

 
L100A
L100B
Protocol

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