Andy Fluor™ 647 Streptavidin

Introduction

Andy Fluor™ 647 streptavidin is prepared by covalently labeling streptavidin with Andy Fluor™ 647 dye. Streptavidin has high binding affinity with biotin, so streptavidin conjugate is commonly used together with biotin conjugate for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules. Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays.

Reconstitution and Storage: Store lyophilized power at -20ºC. When ready to use, rehydrate with dH2O (50 µL for 100 µg streptavidn or 250 µL for 500 µg streptavidn) to make 2 mg/mL solution and centrifuge if not clear. Product is stable for about 6 months at 2-8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.

Form Information  

Immunofluorescent analysis of α-tubulin with Andy Fluor 647streptavidin

 

Physical State: Lyophilized power  
Shipping Condition: Ambient  
Buffer: PBS, pH 7.4, with 0.1% BSA  
Preservative: 0.02% sodium azide  
Storage Conditions: -20ºC, store in dark  
Label: Andy Fluor 647  
Excitation/Emission: 650/665 nm
Unit Size: 100 µg, 500 µg
   
Applications Dilution
Western Blot (WB) 1:5,000 – 1:20,000
Immunofluorescence (IF) 1:100 – 1:2,000
Immunocytochemistry (ICC) 1:100 – 1:2,000
Immunohistochemistry (IHC) 1:100 – 1:2,000
Flow Cytometry (FACS) 1:100 – 1:1,000
   

To Order

Buy Cat.No. Product name Ex/Em (nm) Size Price(¥)

L159A
L159B
Andy Fluor™ 647 Streptavidin 650/665 100 µg
500 µg
300
600

 
L159A
L159B
Protocol