The interaction between biotin and avidin or streptavidin has been exploited for use in many protein and nucleic acid detection and purification methods. Because the biotin label is stable and small, it rarely interferes with the function of labeled molecules enabling the avidin-biotin interaction to be used for the development of robust and highly sensitive assays. The avidin-biotin detection system allows an almost unlimited number of primary detection reagents (i.e., antibodies, nucleic acid probes and ligands) to be easily captured, immobilized or detected with secondary detection reagents generated by modifying avidin or streptavidin. Biotin Derivatives are employed in biotechnology as biotinylation reagents. Applied BioProbes offers many biotin reagents to facilitate biotinylation in the lab. Biotin Derivative Selection Guide
Figure 1. Application of biotin derivatives
Cat. No.
Product Name
Application
Unit Size
C403A
Biotin-11-dUTP, 1 mM in TE buffer
TUNEL, FISH
50 µL
C403B
Biotin-11-dUTP, lyophilized powder
TUNEL, FISH
50 nmol
C404A
Biotin-11-UTP, 1 mM in TE buffer
TUNEL, FISH
50 µL
C404B
Biotin-11-UTP, lyophilized powder
TUNEL, FISH
50 nmol
A031
Biotin-X Annexin V, 100 assays
Apoptosis
25 µg
C303
Biotin Alkyne
Click labeling
5 mg
C304
Biotin Azide
Click labeling
5 mg
C325
Biotin Picolyl Azide
Click labeling
1 mg
C330
Biotin DBCO
Click labeling
1 mg
L020
iLink™ Biotin Antibody Labeling Kit
Antibody labeling
3 labeling
B001
Biotin
Biotinylation reagent
1 g
B002
Biotin
Biotinylation reagent
5 g
B003
Biotin, SE
Biotinylation reagent
100 mg
B004
Biotin-X, SE
Biotinylation reagent
100 mg
B005
Biotin-XX, SE
Biotinylation reagent
50 mg
B006
Biotin-PEG3, SE
Biotinylation reagent
25 mg
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