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Caspase-3/7 Z-DEVD-R110 HTS Assay Kit

产品介绍

The Caspase-3/7 Z-DEVD-R110 HTS Assay Kit is specifically designed for HTS-based assays. The kit provides a single-step homogenous assay system for fast and highly sensitive detection of caspase-3/7 activity. The kit can be used to continuously measure the activity of caspase-3/7 in cell extracts and purified enzyme preparations, using a fluorometer or fluorescence microplate reader.
The rhodamine 110-derived substrate (Z-DEVD-R110) used in this assay is a non-fluorescent bisamide compound that, upon enzymatic cleavage, is converted in a two-step process to the fluorescent monoamide and then to the even more fluorescent R110 product. Both of these hydrolysis products exhibit spectral properties similar to those of fluorescein, with peak excitation and emission wavelengths of 496 nm and 520 nm, respectively.
In addition to the Z-DEVD–R110 substrate, the Caspase-3/7 Z-DEVD-R110 Assay Kit contains the reversible aldehyde inhibitor Ac-DEVD-CHO, as well as the reference standard R110. The Ac-DEVD-CHO inhibitor confirms that the fluorescence signals in both induced and control cell populations are due to the activity of caspase-3/7. The reference standard allows for quantification of the amount of R110 released in the reaction.

产品特点:

  • HTS-compatible: Single-step homogenous assay specifically designed for HTS-based detection.
  • Fast: Fast enzyme kinetics.
  • Sensitive: Green fluorescent readout.

 

产品特性

检测平台: Microplate Reader, Fluorometer
检测方法: Fluorescent
Ex/Em: 496/520 nm
包装规格: 100 assays
储存条件: -20℃,避光
运输条件: 干冰运输

产品应用

细胞凋亡分析

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 
 
 

订购

购买 货号 产品名称 激发/发射波长 规格 价格(¥)
A045 Caspase-3/7 Z-DEVD-R110 HTS Assay Kit 496/520 100 assays 2600

Protocol
   

文档资料

   
产品说明书 (PDF): A045