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eLuminol™ Protein Gel Stain

产品介绍

eLuminol™ Protein Gel Stain is a high sensitive fluorescent stain designed for detecting proteins in SDS polyacrylamide gels. It is as sensitive as silver stain. Unlike silver stain, however, eLuminol™ Protein Gel Stain has a linear detection range of at least 3 orders of magnitude. Protein gel staining with eLuminol™ is a very simple and fast protocol. It doesn’t require fixation step, only need one simple staining step with 1X eLuminol™ for 90 minutes or less, followed by a simple destaining step. Protein gel staining with eLuminol™ is compatible with downstream protein analyses such as mass spectrometry and Edman-based sequencing.
eLuminol™ Protein Gel Stain is available as a 1,000X concentrated solution, which can be readily diluted to the 1X working solution using methanol/acetic acid/water solution. It is very convenient for shipping and storage. The price of eLuminol™ Protein Gel Stain is comparable to the commercial Coomassie® Blue-based protein gel staining solutions, while eLuminol™ provides far better sensitivity and user-friendliness.

Features

  • High sensitivity: ~0.5 ng protein
  • Compatibility with standard laboratory equipment
  • Wide linear detection range: At least three orders of magnitude
  • Compatible with downstream analysis: Compatible with MS and sequencing
  • Stable: Stable at room temperature for 1 year

Applications

Protein gel stain

 

 

 

 

 

 

 

 

 

 

 

 

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Comparison eLuminol with SYPRO Ruby

Protocol Step SYPRO Ruby eLuminol
Fixation step 1 15 min, 50% methanol /7.5% acetic acid None
Fixation step 2 15 min, 50% methanol /7.5% acetic acid None
Staining Overnight 90 min
Destaining 30 min, 10% methanol/ 7% acetic acid 20 min, 10% methanol/ 7% acetic acid
Rinse step 1 5 min, water Optional: 5 min, water
Rinse step 2 5 min, water

订购

购买 货号 产品名称 描述 规格 价格(¥)

P003A
P003B
eLuminol™ Protein Gel Stain (1,000X) Fluorescent protein gel stain 500 µL
1 mL
600
1000

 

Protocol MSDS