In Situ BrdU Cell Proliferation Kit

In Situ BrdU细胞增殖分析试剂盒利用抗BrdU抗体来检测在细胞增殖时掺入到新合成DNA中的5-溴脱氧尿嘧啶核苷（BrdU）。第一步，BrdU与细胞一起孵育或注射到动物体内，BrdU能够掺入到增殖细胞新合成的DNA中。然后经过固定、DNA变性处理，加入BrdU抗体来检测掺入的BrdU（这一步中的DNA变性是必须的，这样能够促进BrdU与其抗体的结合。）最后使用荧光标记的二抗来识别BrdU抗体。该试剂盒已经优化应用于荧光显微镜检测。

Figure 1. Detection of cell proliferation in mouse tissue. A mouse was injected intraperitoneally with 10 µg BrdU per kilogram body weight, then sacrificed at 4 hr. The mouse intestine tissue was detected with BrdU mAb, followed by incubation with Andy Fluor™ 488 labeled secondary antibody (green), and counterstained with DAPI (blue).