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Labeled Nucleotides

Introduction

Applied BioProbes offers 5′-aminoallyl dUTP and UTP analogs, as well as conjugates with biotin and various fluorescent dyes, featuring our exceptionally bright and photostable Andy Fluor dyes. Fluorescent and biotin-labeled dUTP and UTP are widely used for enzymatic incorporation into DNA or RNA.

Advantages

  • Bright signals with low background
  • Wide variety of fluorophores.
  • Great for many applications, like Fluorescence in situ Hybridization (FISH; Figure 1), TUNEL (Figure 2), and more.

 

Figure 1. Fluorescence in situ Hybridization (FISH) of CEP7/MET probes labeled with Andy Fluor dyes. Human Chromosome 7 Enumeration (CEP7) and Human c-Met gene (MET) probes were labeled with Andy Fluor 488-X-dUTP (green) and Andy Fluor 568-X-dUTP (magenta), respectively, using nick translation. The labeled probes were mixed together in hybridization buffers with blocking DNA, and incubated with HT1080 cells on slides at 37°C overnight. The slides was washed and mounted with AntiFade solution containing DAPI (blue), then visualized with an Epi-Fluorescence microscope.

TUNEL-biotin-11-UTP mouse tongue

Figure 2. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-mediated detection of apoptotic cells in mouse tongue tissue using Biotin-11-dUTP. Left: Cells were labeled with Biotin-11-dUTP, followed by detection with AndyFluor™ 488-streptavidin (green). Nuclei were also counterstained with DAPI (blue). Right: Cells were labeled with Biotin-11-dUTP, followed by detection with horse radish peroxidase (HRP).

To order

Labeled nucleotides

Buy Catalog # Product Name Ex/Em (nm) Unit Size List Price
C401A 5-Aminoallyl-dUTP, 10 mM in TE buffer 100 µL $120
C401B 5-Aminoallyl-dUTP, lyophilized powder 1 µmol $120
C402A 5-Aminoallyl-UTP, 10 mM in TE buffer 100 µL $110
C402B 5-Aminoallyl-UTP, lyophilized powder 1 µmol $110
C403A Biotin-11-dUTP, 1 mM in TE buffer 50 µL $180
C403B Biotin-11-dUTP, lyophilized powder 50 nmol $180
C404A Biotin-11-UTP, 1 mM in TE buffer 50 µL $180
C404B Biotin-11-UTP, lyophilized powder 50 nmol $180
C405A 5-FAM-dUTP, 1 mM in TE buffer 495/518 25 µL $140
C405B 5-FAM-dUTP, lyophilized powder 495/518 25 nmol $140
C406A 5-FAM-X-dUTP, 1 mM in TE buffer 495/518 25 µL $180
C406B 5-FAM-X-dUTP, lyophilized powder 495/518 25 nmol $180
C407A 5-FAM-UTP, 1 mM in TE buffer 495/518 25 µL $140
C407B 5-FAM-UTP, lyophilized powder 495/518 25 nmol $140
C408A 5-FAM-X-UTP, 1 mM in TE buffer 495/518 25 µL $180
C408B 5-FAM-X-UTP, lyophilized powder 495/518 25 nmol $180
C409A Andy Fluor 488-X-dUTP, 1 mM in TE buffer 500/525 25 µL $280
C409B Andy Fluor 488-X-dUTP, lyophilized powder 500/525 25 nmol $280
C410A Andy Fluor 488-X-UTP, 1 mM in TE buffer 500/525 25 µL $280
C410B Andy Fluor 488-X-UTP, lyophilized powder 500/525 25 nmol $280
C411A Andy Fluor 555-X-dUTP, 1 mM in TE buffer 553/565 25 µL $280
C411B Andy Fluor 555-X-dUTP, lyophilized powder 553/565 25 nmol $280
C412A Andy Fluor 555-X-UTP, 1 mM in TE buffer 553/565 25 µL $280
C412B Andy Fluor 555-X-UTP, lyophilized powder 553/565 25 nmol $280
C413A Andy Fluor 568-X-dUTP, 1 mM in TE buffer 578/602 25 µL $280
C413B Andy Fluor 568-X-dUTP, lyophilized powder 578/602 25 nmol $280
C414A Andy Fluor 568-X-UTP, 1 mM in TE buffer 578/602 25 µL $280
C414B Andy Fluor 568-X-UTP, lyophilized powder 578/602 25 nmol $280
C415A Andy Fluor 594-X-dUTP, 1 mM in TE buffer 590/615 25 µL $280
C415B Andy Fluor 594-X-dUTP, lyophilized powder 590/615 25 nmol $280
C416A Andy Fluor 594-X-UTP, 1 mM in TE buffer 590/615 25 µL $280
C416B Andy Fluor 594-X-UTP, lyophilized powder 590/615 25 nmol $280
C417A Andy Fluor 647-X-dUTP, 1 mM in TE buffer 650/665 25 µL $280
C417B Andy Fluor 647-X-dUTP, lyophilized powder 650/665 25 nmol $280
C418A Andy Fluor 647-X-UTP, 1 mM in TE buffer 650/665 25 µL $280
C418B Andy Fluor 647-X-UTP, lyophilized powder 650/665 25 nmol $280
C419A Cy3-X-dUTP, 1 mM in TE buffer 553/565 25 µL $240
C419B Cy3-X-dUTP, lyophilized powder 553/565 25 nmol $240
C420A Cy3-X-UTP, 1 mM in TE buffer 553/565 25 µL $240
C420B Cy3-X-UTP, lyophilized powder 553/565 25 nmol $240
C421A Cy5-X-dUTP, 1 mM in TE buffer 650/665 25 µL $240
C421B Cy5-X-dUTP, lyophilized powder 650/665 25 nmol $240
C422A Cy5-X-UTP, 1 mM in TE buffer 650/665 25 µL $240
C422B Cy5-X-UTP, lyophilized powder 650/665 25 nmol $240