产品介绍
慢病毒包装试剂盒
GeneCopoeia的Lenti-Pac™ HIV和FIV表达包装系统包括优化的慢病毒包装质粒,eGFP阳性对照质粒,转染试剂,增加病毒产量的EndoFectin™转染试剂和TiterBoost™滴度增强剂。该系统与GeneCopoeia的ORF、shRNA、miRNA及Promoter克隆配套使用保证更高滴度和更高表达水平。
HIV和FIV包装系统中的包装质粒经过设计调整,确保将重组子包装成具有高感染率的慢病毒颗粒,同时防止了病毒的自我复制。
293Ta慢病毒包装细胞系
293Ta包装细胞系是常用于病毒包装的工具细胞。Lenti-Pac™ 293Ta包装细胞系转染效率高,能够实现病毒蛋白高水平的表达。
慢病毒浓缩试剂
Lenti-Pac™ 慢病毒浓缩试剂是GeneCopoeia为用户设计的用于快速、简便浓缩慢病毒颗粒的慢病毒浓缩试剂。实验操作时只需将浓缩试剂与需浓缩的慢病毒上清液混合,经短暂孵育,普通离心机离心即可。
慢病毒滴度检测试剂盒
GeneCopoeia设计的Lenti-Pac™ HIV和FIV qRT-PCR慢病毒滴度检测试剂盒可快速、简便地测定病毒拷贝数,并确定病毒包装是否成功。该试剂盒主要组分包括RNA抽提试剂, qRT-PCR反应试剂以及内参对照。慢病毒的RNA基因组被反转录后用基于SYBR® Green I原理的qRT-PCR技术进行定量。
相关产品
- 慢病毒载体的克隆 — ORF、shRNA、miRNA、miRNA抑制剂以及Promoter克隆
- 预制慢病毒颗粒 — iPSC基因、ORF克隆、阳性和阴性对照等
- 特殊慢病毒包装服务 — 价格低且周期短,包含ORF,miRNA或miRNA inhibitors序列的慢病毒载体
- 客户定制的慢病毒服务 — 40多种载体和多种融合标签,可定制任意ORF,shRNA或miRNA载体
发表的文章
- Hunter, K. et al (2013) Inherited variation in miR-290 expression suppresses breast cancer progression by targeting the metastasis susceptibility gene Arid4b. Cancer Research, doi: 10.1158/0008-5472.CAN-12-3513. [Lenti-Pac™ FIV Expression Packing Kit].
- Hirata, H. et al (2013) MicroRNA-182-5p Promotes Cell Invasion and Proliferation by Down Regulating FOXF2, RECK and MTSS1 Genes in Human Prostate Cancer. PloS one, 8(1): e55502. doi:10.1371/journal.pone.0055502. [Lenti-Pac™ HIV Expression Packing Kit].
- Sanchez-Alvarez, R. at al (2013) Mitochondrial dysfunction in breast cancer cells prevents tumor growth: Understanding chemoprevention with metformin. Cell Cycle, Volume 12, Issue 1, January 1, 2013 Pages 172-182. [Lentiviral plasmids, packaging cells and reagents].
- Musick, J. et al. (2012) Induced Pluripotent Stem Cells and Related Methods. U.S. Patent No. 20,120,276,070. 1 Nov. 2012. [Lenti-Pac™ HIV Expression Packing Kit].
- Amodio, N. et al. (2012) miR-29b sensitizes multiple myeloma cells to bortezomib-induced apoptosis through the activation of a feedback loop with the transcription factor Sp1. Cell Death and Disease, 3, e436; doi:10.1038/cddis.2012.175. [Lenti-Pac™ FIV Expression Packaging Kit].
- Simpfendorfer, K. et al. (2012) The autoimmunity-associated BLK haplotype exhibits cis-regulatory effects on mRNA and protein expression that are prominently observed in B cells early in development. Hum. Mol. Genet. (2012) 21 (17): 3918-3925. doi: 10.1093/hmg/dds220. [HIV Lenti-Pac™].
- Yamamura, S. et al. (2012) MicroRNA-34a suppresses malignant transformation by targeting c-Myc transcriptional complexes in human renal cell carcinoma. Carcinogenesis (2012) 33 (2): 294-300. doi: 10.1093/carcin/bgr286 . [HIV Lenti-Pac].