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QuantiTaq™ Thermostable DNA Polymerase

   
QuantiTaq™ DNA Polymerase is an ultra-pure, thermostable DNA Polymerase purified through many steps of optimized chromatography. Our production and quality control processes ensure utmost purity, reproducibility, and processivity. The enzyme meets the requirements of specialized PCR with a linear amplification manner and highly reproducible when a template is unlimited diluted. QuantiTaq™ DNA Polymerase is packaged with 10X Reaction Buffer and Dilution Buffer.
Application: DNA amplification via PCR; Primer extension for probe labeling; RT-PCR; AmpArray™ gene expression profiling.Features: High purity; Ultra reproducible results; Robotic use.

Features: High purity; Ultra reproducible results; Robotic use.

Storage Buffer: 20 mM Tris-HCl pH 8.0 , 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol, and 0.5% Tween 20 and other integrated components for stability of long time storage.

Dilution buffer: 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, and other integrated components for precise and easy aliquot to assemble reactions.

 
 
Fig. 1. Amplification of human G3PDH with first strand cDNA from T-cell by QuantiTaq™ and various Taq DNA polymerases from other suppliers.
The total RNA (10µg) was transcribed by reverse transcriptase in 100 µl reaction mixture. After diluted 5, 25, 125, 625, 3125 and 15625 fold (as shown in wells of columns 1, 2, 3, 4, 5, and 6 above, respectively), 2µl of diluted reaction mixture was added to the 23µl of PCR reaction mixture containing indicated Taq DNA polymerases. PCR was performed: 94°C for 3' followed by 30 cycles (94°C for 30", 62°C for 30" 72°C for 45").
Reaction Buffer:10x Reaction Buffer with Mg2+: 500 mM KCl, 100 mM Tris-HCl pH 8.5 (at 25°C), 15mM Mg++ and other integrated components for robotic use.Unit Definition: One unit of either enzyme will incorporate 10 nmol of total dNTPs in 30 minutes at 72°C.

 

Associated Activities: Endonuclease, exonuclease, and DNA ligation activities were not detectable after one or two hours of incubation time, respectively, of 1 µg Lamda DNA and 0.22 µg of EcoRI-digested Lamda DNA at 72°C in the presence of 15-20 units of QuantiTaq™ DNA Polymerase.